The antagonistic effect of dexamethasone and insulin on alpha-fetoprotein secretion by cultured H4-II-E-C3 cells derived from the Reuber H-35 hepatoma. |
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Authors: | Y Tsukada W L Richards J E Becker V R Potter H Hirai |
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Institution: | 1. McArdle Laboratory for Cancer Research, Madison, Wisconsin 53706 USA;2. Department of Biochemistry, Hokkaido University School of Medicine, Sapporo 060 Japan |
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Abstract: | Non-confluent monolayers of H4-II-E-C3 cells were maintained in serum-free media. Dexamethasone alone (5 × 10?7M) stimulated α-fetoprotein secretion 2- to 4-fold while insulin alone (8.7 × 10?8M) inhibited α-fetoprotein secretion by 20%. When dexamethasone (5 × 10?7 to 5 × 10?9M) and insulin (8.7 × 10?8 to 8.7 × 10?11M) were added simultaneously, insulin diminished the stimulatory effect of dexamethasone. When α-fetoprotein secretion was elevated by dexamethasone and the medium was replaced by media containing either insulin or no hormones, the rate of α-fetoprotein secretion diminished more rapidly with the insulin-supplemented medium. Alone or in combination, insulin and dexamethasone had little effect on albumin secretion. |
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Keywords: | INS insulin DEX dexamethasone ALB albumin AFP α-fetoprotein |
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