Identification of kinin-related peptides in the disease vector, Rhodnius prolixus |
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Authors: | Te Brugge Victoria Paluzzi Jean-Paul Neupert Susanne Nachman Ronald J Orchard Ian |
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Institution: | a Department of Biology, University of Toronto Mississauga, 3359 Mississauga Road North, Mississauga, Ontario L5L 1C6, Canada b Areawide Pest Management Research, Southern Plains Agricultural Research Center, USDA, 2881 F/B Road, College Station, TX 77845, USA c Institute of Zoology, Friedrich-Schiller-University, Erbertstrasse 1, Jena 07743, Germany |
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Abstract: | We have used an in silico approach to identify a gene from the blood-gorging vector, Rhodnius prolixus, that is predicted to produce an insect kinin prepropeptide. The prepropeptide is 398 amino acids in length and can potentially produce a large number of kinin-related peptides following post-translational processing. A comparison with other insect kinin precursor sequences demonstrates greatest conservation at the C-terminal region of the kinin peptides. Multiple peptides predicted from the kinin gene are phenotypically expressed in R. prolixus, as revealed by MALDI-TOF MS MS, including 12 kinins and one kinin precursor peptide (KPP). Six of these peptides are characterized by the typical insect kinin C-terminal motif FX1X2WGamide and five of these are also found as truncated forms. Five peptides were identified with an atypical, though similar, FX1X2WAamide C-terminus. There is also peptide with a C-terminal DDNGamide motif and a number of non-amidated peptides. |
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Keywords: | In silico analysis Kinin gene MALDI-TOF MS MS Chagas&rsquo disease Insect |
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