Effects of Gibberellic Acid and Abscisic Acid on Shoot Formation in Tobacco Callus Cultures |
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| Authors: | TREVOR A THORPE DONALD D MEIER |
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| Institution: | Department of Biology, University of Calgary, Calgary, Alberta, Canada T2N 1N4 |
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| Abstract: | Tobacco (Nicotiana tabacum L. cv. W. 38) callus grown on a shoot-forming medium was exposed to gibberellic acid (GA3) and abscisic acid (ABA) for varying lengths of time and at different periods during culture. The results suggest that if the tissue accumulated sufficient GA3 prior to the initiation of meristemoids and shoot primordia, repression of shoot formation occurred. This repression was not reversed by increasing the levels of auxin or cytokinin in the medium, but ABA could partially overcome the GA3 repression of shoot formation. |
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