Efficient plant regeneration from shoot apices of sorghum |
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| Authors: | M Maheswari N Jyothi Lakshmi S K Yadav Y Varalaxmi A Vijaya Lakshmi M Vanaja B Venkateswarlu |
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| Institution: | (1) Division of Crop Sciences, Central Research Institute for Dryland Agriculture, Santosh Nagar, Hyderabad, 500 059, Andhra Pradesh, India |
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| Abstract: | An efficient and rapid regeneration protocol was developed using shoot apices from germinating seedlings of two cultivars
of sorghum, SPV-462 and M35-1, as explants. A vertical slit given from the base of each dissected apex enhanced the efficiency
of callusing response by two fold. MS medium containing 0.5 mg dm−3 each of 2,4-D and kinetin was most effective in producing friable and embryogenic calli. Scanning electron microscopy of
these calli detected somatic embryogenesis. Calli thus induced gave rise to approximately 42 green shoots per callus in both
the genotypes when transferred to regeneration medium containing 1.5 mg dm−3 kinetin. |
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| Keywords: | 2 4-dichlorophenoxyacetic acid kinetin plant growth regulators Sorghum bicolor (L ) Moench |
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