两株枯草芽孢杆菌的噬菌体

从三门峡酶制剂厂分离到与过去形态不同的两种噬菌体BS3l和BS32。BS31有收缩尾鞘，头部为六边形；BS32有复杂的短尾，形态与φ29相似，寄主范围窄且有差异，用限制酶分析，噬菌体DNA的分子量分别为62kb和17kb。根据解链温度计算噬菌体DNA的G十c含量分别为45．7mol％和40．7mol％。两株噬菌体的结构蛋白经SDS聚丙烯酰胺凝胶电沫测定，BS3l有2条主带，10条次带；BS32有3条主带和6条次带。

Properties of two Bacillus subtilis bacteriophages

Two lytic bacteriophages of Bacillus subtilis BF7658 were isolated. Their morphology, biological properties and physiochemical characteristics of their DNAs were compared. Electromicroscopic observation indicated that BS31 has a hexagonal head and contracted tail sheath, BS32 is a complex short-tailed phage and similar to phi 29 but differs from phi 29 in size and other properties. Two phages have a narrow spectrum of host range. The molecular weight of BS31 and BS32 DNAs are 62kb and 17kb respectively by EcoR1 endonuclease analysis. The G + C content of the DNAs are 45.7%(BS31) and 40.7%(BS32). Structural proteins of BS31 gave two major bands and at least ten minor ones; BS32 gave three major bands and six minor ones by SDS-polyacralamide gel electrophoresis.