Optimization of submerged culture conditions for the production of angiotensin converting enzyme inhibitor from <Emphasis Type="Italic">Flammulina velutipes</Emphasis> |
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| Authors: | J M Kim K S Ra D O Noh H J Suh |
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| Institution: | (1) Department of Food and Nutrition, College of Health Sciences, Korea University, Seoul 136-703, South Korea, KR;(2) Department of Food and Nutrition, Taegu Technical College, Taegu 704-721, South Korea, KR;(3) School of Human Ecology, Kaya University, Koryong, 717-800, South Korea, KR |
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| Abstract: | The angiotensin-converting enzyme (ACE) inhibitory effect was tested in the culture broth from submerged mycelial cultures
of 20 basidiomycetes. The ACE inhibitory effect of culture broth from Flammulina velutipes strain 414 was the highest (52.8%), followed by Lentinus edodes strains 2 (44.4%) and 16 (41.3%). Nutritional requirements for the production of ACE inhibitory substance from F. velutipes were studied. Sucrose, ammonium acetate, and glutamic acid were chosen for the maximum production of ACE inhibitory substance.
The optimal medium composition was (g/l): sucrose 20, ammonium acetate 5, glutamic acid 2, KH2PO4 3, MgSO4·7H2O 0.8, and yeast extract 0.5. Under optimal culture conditions, the ACE inhibitory effect was more than 80%.
Received 04 May 2002/ Accepted in revised form 11 June 2002 |
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| Keywords: | : Flammulina velutipes submerged fermentation optimization ACE inhibitor |
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