Eimeria papillata: upregulation of specific miRNA-species in the mouse jejunum |
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| Authors: | Dkhil Mohamed Abdel-Baki Abdel Azeem Delić Denis Wunderlich Frank Sies Helmut Al-Quraishy Saleh |
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| Institution: | aDepartment of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;bDepartment of Zoology and Entomology, Faculty of Science, Helwan University, Egypt;cDepartment of Zoology, Faculty of Science, Beni-Suef University, Egypt;dDepartment of Molecular Parasitology, Heinrich Heine University, 40225 Duesseldorf, Germany;eDepartment of Biochemistry and Molecular Biology, Heinrich Heine University, 40225 Duesseldorf, Germany |
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| Abstract: | Increasing evidence indicates miRNAs as critical regulators of gene expression, but little information is available for miRNAs in intestinal diseases. Here, we investigated intestinal infections of male Balb/c mice with the coccidian parasite Eimeria papillata. On day 4 after oral infection, mice were shedding 3150 ± 430 oocysts per gram feces. This was associated with a low inflammatory response of the jejunum of mice evidenced by histology, non-response of IL-1β mRNA, even slight downregulation of IL-6 mRNA, only slight increases in iNOS mRNA, nitrate/nitrate, malondialdehyde, and a small decrease in glutathione, respectively. Only IFNγ mRNA was strongly induced. Using miRNA microarray technology, there were significantly upregulated the four miRNA species miR-1959, MCMV-miR-M23-1-5P, miR-203, and miR-21 out of 634 miRNAs, which was also confirmed by quantitative RT-PCR. Our data provide evidence that E. papillata parasites are able to induce specific miRNA species in their host target organ. |
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| Keywords: | Eimeria papillata miRNA Mouse Jejunum |
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