Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear |
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Authors: | Lee Sun Joo Jeong Hae Jin Lee Ji Eun Lee Jong Soo Xuan Ying Hua Kong Hyun-Hee Chung Dong-Il Ock Mee-Sun Yu Hak Sun |
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Institution: | Department of Parasitology, School of Medicine, Pusan National University, Busan, Korea. |
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Abstract: | In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene biguanide, chlorhexidine and oral itraconazole, which resulted in resolution of the patientos ocular inflammation. The majority of the molecular characteristics of the KA/PE1 were determined to be identical, or quite similar, to those of A. castellanii Ma strain, which had been isolated also from amoebic keratitis. The risk of Acanthamoeba keratitis as a potential complication of overnight orthokeratology is briefly discussed. |
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Keywords: | Acanthamoeba castellanii Ma orthokeratology amoebic keratitis polyhexamethylene biguanide (PHMB) 18S rDNA sequence analysis |
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