The role of light and CO2 in optimising the conditions for shoot proliferation of Actinidia deliciosa in vitro |
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| Authors: | R Infante E Magnanini B Righetti |
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| Institution: | Centro di Studio per la Tecnica Frutticola, C.N.R., via Filippo Re 6, I-40126 Bologna, Italy;Istituto di Coltivazioni Arboree, Univ. di Bologna, via Filippo Re 6, I-40126 Bologna, Italy. |
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| Abstract: | Proliferating cultures of Actinidia deliciosa A. Chev., C. F. Liang and A. R. Ferguson cv. Tomuri (♂) were grown under photosynthetic photon flux density (PPFD) rates ranging from 30 to 250 μmol m?2 s?1 in order to determine certain physiological parameters in vitro: CO2 evolution, photosynthesis at three CO2 atmospheric concentrations (330, 1450 and 4500 μl l?1), fresh and dry matter accumulation and proliferation rate.
A proportional response in dry weight, dry/fresh weight ratios and PPFD was found. The proliferation rate increased up to 120 μmol m?2 s?1 but decreased at higher rates. At the highest PPFD, the CO2 released from cultures and accumulated in the vessels reached 200 μl l?1 of; at the lowest rate the CO2 concentration reached 10500 μl l?1 after 28 days of culture. The photosynthetic rate at 1450 and 4500 μl l?1 of CO2 was nearly 4 times higher than at the lowest concentration tested. |
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| Keywords: | Actinidia deliciosa CO2 evolution in vitro cultures light intensity micropropagation photosynthesis |
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