Exploring potential use of Australian thraustochytrids for the bioconversion of glycerol to omega-3 and carotenoids production |
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| Institution: | 1. Centre for Chemistry and Biotechnology, Geelong Technology Precinct, Deakin University, Geelong 3217, Victoria, Australia;2. Marine Biodiversity and Biosecurity, NIWA, Kilbirnie, Wellington 6021, New Zealand;3. DBT-IOC Centre for Advance Bioenergy Research, Research & Development Centre, Indian Oil Corporation Limited, Sector-13, Faridabad 121007, India;1. DBT-IOC Centre for Advanced Bioenergy Research, Research & Development Centre, Indian Oil Corporation Limited, Sector-13, Faridabad 121007, India;2. Centre for Chemistry and Biotechnology, Waurn Ponds, Deakin University, Victoria 3217, Australia;1. Institute of Mechanical, Process & Energy Engineering, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS, UK;2. CSIRO Oceans and Atmosphere, GPO Box 1538, Hobart, TAS, 7001, Australia;3. Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS, UK;4. Jane L Polglase Institute of Life and Earth Sciences, School of Energy, Geoscience, Infrastructure and Society, Heriot-Watt University, Edinburgh EH14 4AS, UK;1. Centre for Chemistry and Biotechnology, Waurn Ponds, Deakin University, Victoria 3217, Australia;2. DBT-IOC Centre for Advance Bioenergy Research, Research & Development Centre, Indian Oil Corporation Limited, Sector-13, Faridabad 121007, India |
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| Abstract: | Marine microbes have the potential for accumulating large quantities of lipids and are therefore suitable candidate as feedstock in unsaturated fatty acid production. The efficient utilisation of glycerol as an alternative carbon source to glucose was demonstrated in the fermentation of newly isolated thraustochytrid strains from the Queenscliff, Victoria, Australia. The isolates exhibited the presence of omega-3 and omega-6 polyunsaturated fatty acids, with the major fatty acids for all isolates being (as percent total fatty acid), palmitic acid (25.1–40.78%), stearic acid (4.24–13.2%), eicosapentaenoic acid EPA (2.31–8.5%) and docosapentaenoic acid (7.24–10.9%). Glycerol as a carbon source gave promising biomass growth with significant lipid and DHA productivity. An approximate three-fold increase in carotenoid content in all isolates was achieved when glycerol was used as a carbon source in the production medium. |
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| Keywords: | β-Carotene Marine microalgae Astaxanthin Canthaxanthin Biofuel |
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