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Quantitative determination of pectic substances as an example of a rhamnopolysaccharide assay
Authors:P L Turecek  E Buxbaum  F Pittner
Affiliation:Institut für Allgemeine Biochemie der Universit?t Wien, Austria.
Abstract:A quantitative enzymatic assay for rhamnopolysaccharides is described. The procedure is shown with pectic substances as an example. The test is based on the enzymatic degradation of the macromolecules to liberate L-rhamnose. This sugar can be quantitatively determined with the help of L-rhamnose dehydrogenase under concomitant reduction of NAD, thus allowing the quantitative evaluation of the original pectin.
Keywords:
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