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Antisera-mediated in vivo reduction of Cry1Ac toxicity in Helicoverpa armigera |
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| Authors: | Chenxi Liu Changming Ning Kongming Wu Brenda Oppert |
| Affiliation: | a State Key Laboratory of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, West Yuanmingyuan Road, Beijing 100193, China b Department of Entomology, College of Agronomy and Biotechnology, China Agricultural University, West Yuanmingyuan Road, Beijing 100193, China c USDA ARS Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA |
| Abstract: | A functional assessment of Bacillus thuringiensis (Bt) toxin receptors in the midgut of lepidopteran insects will facilitate understanding of the toxin mode of action and provide effective strategies to counter the development of resistance. In this study, we produced anti-aminopeptidase (APN) and anti-cadherin sera with purified Cry1Ac toxin-binding APN or cadherin fragments from Heliocoverpa armigera. Antisera were evaluated for their effects on Cry1Ac toxicity through bioassays. Our results indicated that both the anti-APN and anti-cadherin sera reduced Cry1Ac toxicity in vivo, although cadherin antiserum reduced toxicity more than APN antiserum. These results suggest that both APN and cadherin are involved in Cry1Ac intoxication of H. armigera, evidence that the pore formation model may be representative of Cry1Ac toxin mode of action in this insect. |
| Keywords: | Antiserum Bacillus thuringiensis Aminopeptidase N Cadherin Cry1Ac toxin Toxicity |
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