Comparative study of Bacillus thuringiensis Cry1Ia and Cry1Aa delta-endotoxins: Activation process and toxicity against Prays oleae |
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Authors: | Mariam Dammak Souad Rouis |
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Institution: | a Laboratory of Biopesticides, Centre of Biotechnology of Sfax, Tunisia, P.O. Box 1177, 3061 Sfax, Tunisia b Biological & Environmental Sciences Department, College of Arts and Sciences, Qatar University, P.O. Box 2713, Doha, Qatar |
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Abstract: | Cry1Ia and Cry1Aa proteins exhibited toxicities against Prays oleae with LC50 of 189 and 116 ng/cm2, respectively. The ability to process Cry1Ia11 protoxin by trypsin, chymotrypsin and P. oleae larvae proteases was studied and compared to that of Cry1Aa11. After solubilization under high alkaline condition (50 mM NaOH), Cry1Aa11 was converted into a major fragment of 65 kDa, whereas Cry1Ia11 protoxin was completely degraded by P. oleae larvae proteases and trypsin and converted into a major fragment of 70 kDa by chymotrypsin. Using less proteases of P. oleae juice, the degradation of Cry1Ia11 was attenuated. When the solubilization (in 50 mM Na2CO3 pH 10.5 buffer) and activation were combined, Cry1Ia11 was converted into a proteolytic product of 70 kDa after 3 h of incubation with trypsin, chymotrypsin and P. oleae juice. These results suggest that the in vivo solubilization of Cry1Ia11 was assured by larval proteases after a swelling of the corresponding inclusion due to the alkalinity of the larval midgut. |
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Keywords: | Bacillus thuringiensis P oleae Cry1Aa11 Cry1Ia11 Solubilization Activation Proteolysis Toxicity Trypsin Chymotrypsin Larvae juice |
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