| B群脑膜炎奈瑟氏球菌铁调蛋白的诱导表达及其性质研究 |
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| 引用本文： | 尹翠凤,王立亚.B群脑膜炎奈瑟氏球菌铁调蛋白的诱导表达及其性质研究[J].微生物学免疫学进展,1997,25(1):16-19. |
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| 作者姓名： | 尹翠凤 王立亚 |
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| 作者单位： | 卫生部北京生物制品研究所 |
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| 摘 要： | 对２株Ｂ群脑膜炎奈瑟氏球菌的铁调蛋白的表达及其性质作了研究。在流脑半综合培养基中加人铁螯合剂，诱导了铁调蛋白的表达，并比较了三种铁螯合剂的效果。结果表明，ＥＤＤＨＡ是最令人满意的螯合剂。ＥＬＩＳＡ实验证实含有铁调蛋白的外膜蛋白可以增强对小白鼠的免疫原性。Ｗｅｓｔｅｒｎ－ｂｌｏｔ实验证明有两种铁调蛋白是有效的抗原成分，分子量分别在７５ｋＤａ和８５ｋＤａ附近。由分子量来推算，分别应为ＦｅＲＰ－７０及ＴＢＰ２。
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| 关 键 词： | 脑膜炎奈瑟氏球菌 铁调蛋白 转铁结合蛋白及转铁结合蛋白2 菌苗 |
Studies on Expression and Characteristics of Iorn Regulated Proteins of Group B Neisseria Meningitidis |
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| Yin Cuifeng,Wang Liya,Tang Qiaoying.Studies on Expression and Characteristics of Iorn Regulated Proteins of Group B Neisseria Meningitidis[J].Progress In Microbiology and Immunology,1997,25(1):16-19. |
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| Authors: | Yin Cuifeng Wang Liya Tang Qiaoying |
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| Abstract: | Our research here was focused on the expression and characteristics of the iron regulated proteins (IRPs) of two Neisseria meningitidis strains,which belong to group B.We induced the expression of IRPs with the addition of iron chelators in our semi synthetic media(SSM)and compared the effects of three kinds of chelators.EDDHA was shown to be most satisfactory.ELISA confirmed the imunogenicity of IRPs.And Western blot assay proved that two kinds of IRPs made this cotribution.Deduced from their molecular weights,one might be TBP2 and the other FeRP-70. |
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| Keywords: | Neisseria meningitidis Iron regulated proteins Transferrin binding protein 1 and 2 Vaccine |
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