| 丙型肝炎病毒核酸疫苗的免疫效果观察 |
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| 引用本文： | 周旭 李益民. 丙型肝炎病毒核酸疫苗的免疫效果观察[J]. 微生物学免疫学进展, 1997, 25(4): 28-33 |
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| 作者姓名： | 周旭 李益民 |
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| 作者单位： | 卫生部兰州生物制品研究所 |
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| 摘 要： | 将丙型肝炎病毒Ｃ＋Ｅ１区基因克隆到不同的真核细胞表达载体ｐＣＤＮＡ３．１（＋）和ｐＳＶＬ中，通过肌肉注射和皮下注射免疫ＢＡＬＢ／Ｃ及Ｆ１小鼠后，产生了抗ＨＣＶ／Ｃ区抗体。其中核酸疫苗ｐｃＤＮＡ３．１－ＨＣＶ／Ｃ＋Ｅ１的免疫高峰的出现早于ｐＳＶＬ－ＨＣＶ／Ｃ＋Ｅ１，Ｆ１小鼠的抗体应答强于ＢＡＬＢ／小鼠。肌肉注射优于皮下注射。
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| 关 键 词： | 丙型肝炎病毒 真核细胞表达载体 基因重组 核酸疫苗 |
Evalution of HCV DNA-mediated immune effect |
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| Abstract: | HCV/C+E1 gene was inserted into two eukaryotic expression vectors pSVL and pcDNA3.1(+),The recombinant plasmids pSVL-HCV/C+E1 and pcDNA-HCV/C+E1 were immunized to BALB/c and F1 mice by muscular and subcutaneous injection and produced positive antibody responses.Aftetr immunization,the antibody peak of pcDNA-HCV/C+E1 arise earlier than pSVL-HCV/C+E1,the immune effect of F1 mice is better than BALB/c mice,the antibody response of the mice immunized by muscular injection is higher than subcutaneous injection. |
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| Keywords: | HCV Eukaryotic expression vector Recombinant plasmid DNA Vaccine |
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