Growth of Bacillus fastidiosus on glycerol and the enzymes of ammonia assimilation |
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Authors: | C van der Drift R A M M Smits G A M Michiels H J M Op den Camp |
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Institution: | (1) Department of Microbiology, Faculty of Science, University of Nijmegen, Toernooiveld, NL-6525 Nijmegen, The Netherlands |
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Abstract: | Bacillus fastidiosus was able to grow on glycerol as a carbon source when allantoin or urate was used as nitrogen source. The primary assimilatory enzyme for glycerol was glycerol kinase; glycerol dehydrogenase could not be detected. The glycerol kinase activity was increased 30-fold in allantoin/glycerol-grown cells as compared to alantoin-grown cells. Under both growth conditions high levels of glutamate dehydrogenase were found. Glutamine synthetase and glutamate synthase activities could not be demonstrated, while low levels of alanine dehydrogenase were present. It is concluded that B. fastidiosus assimilates ammonia by the NADP-dependent glutamate dehydrogenase.Abbreviations GS
glutamine synthetase
- GOGAT
glutamate synthase
- GDH
glutamate dehydrogenase
- ADH
alanine dehydrogenase |
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Keywords: | Bacillus fastidiosus Growth on glycerol Glycerol kinase Ammonia assimilation Glutamate dehydrogenase |
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