| 腺苷产生菌Xn151菌株的选育及发酵条件研究 |
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| 引用本文： | 施庆珊,林小平,许虹,疏秀林,冯静,陈爱美,邱玉棠,欧阳友生,陈仪本.腺苷产生菌Xn151菌株的选育及发酵条件研究[J].生物技术通报,2008(Z1). |
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| 作者姓名： | 施庆珊 林小平 许虹 疏秀林 冯静 陈爱美 邱玉棠 欧阳友生 陈仪本 |
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| 作者单位： | 广东省微生物研究所广东省微生物应用新技术公共实验室,广州,510070 |
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| 基金项目： | 广州市科技计划项目(2006Z3-E0601);;广东省科技攻关项目(2002C1040103)资助 |
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| 摘 要： | 以肌苷产生菌枯草杆菌GMI-741(Ade~(--))为出发菌株,通过多因子诱变选育出具有黄嘌呤、鸟嘌呤双重营养缺陷型、丧失腺嘌呤脱氨酶的腺苷产生菌Xn151。以Xn151为发酵菌株,采用正交实验设计法对其发酵基础培养基进行优化,确定最佳发酵配方。利用此培养及配方摇瓶发酵72h,腺苷产量可达12.3g/L。
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| 关 键 词： | 腺苷 菌种选育 发酵 正交实验设计 |
Breeding of and Fermentation an Adenosine-producing Strain Xn151 |
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| Shi Qingshan,Lin Xiaoping,Xu Hong,Shu Xiulin,Feng Jing,Chen Aimei,Qiu Yutang,OuYang Yousheng,Chen Yiben.Breeding of and Fermentation an Adenosine-producing Strain Xn151[J].Biotechnology Bulletin,2008(Z1). |
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| Authors: | Shi Qingshan Lin Xiaoping Xu Hong Shu Xiulin Feng Jing Chen Aimei Qiu Yutang OuYang Yousheng Chen Yiben |
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| Institution: | Guangdong Institute of Microbiology;Guangdong Open Laboratory of Applied Microbiology;Guangzhou 510070 |
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| Abstract: | The induction of adenosine-producing strain from an inosine-producing strain Bacillus substilis GMI-741 was attempted,and it was found out that a diauxotrophi mutant(xanthine and guanine-requiring)Bacillus substilis Xn151 lacking of adenase produce a large amount of adenosine.The orthogonal factorial design was carried out,to optimize the culture medium.The optimal medium,based on statistical analysis,showed a production of 12.3 g/L adenosine on a reciprocal shaker for 72 h. |
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| Keywords: | Adenosine Strain breeding Fermentation Orthogonal factorial design |
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