Somatic embryogenesis and plant regeneration in tissue cultures of radish (Raphanus sativus L.) |
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Authors: | Won J Jeong Sung R Min Jang R Liu |
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Institution: | (1) Bioresources Research Group, Genetic Engineering Research Institute, KIST, P.O. Box 115, 305-600 Taedok Science Town, Taejon, Korea |
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Abstract: | Hypocotyl segments of 2- to 3-week-old radish (Raphanus sativus L. cv. F1 Handsome Fall) seedlings produced yellowish compact calli when cultured on Murashige and Skoog's (MS) medium supplemented with 1 mgl-1 2,4-dichlorophenoxyacetic acid (2,4-D). Upon transfer onto medium containing 6-benzyladenine and -naphthaleneacetic acid, up to 5.3% of the calli gave rise to a few somatic embryos. When subcultured for 3 to 6 months, 7% of the yellowish, compact calli produced white, compact calli which formed numerous embryos. These calli maintained their embryogenic capacity for over 18 months. When cultured on medium containing 0.1 to 3 mgl-1 2,4-D, up to 90% of longitudinally sliced somatic embryo halves produced calli with numerous secondary embryos. Embryos were transferred onto medium containing 0.1 mgl-1 2,4-D and 1 mgl-1 abscisic acid where they developed into the cotyledonary stage. Upon transfer onto half-strength MS basal medium, approximately 90% of the embryos developed into plantlets. These plantlets were successfully transplanted in potting soil and after cold treatment they were grown to maturity in a phytotron.Abbreviation 2,4-D
2,4-dichlorophenoxyacetic acid
- BA
6-benzyladenine
- GA3
gibberellin A3
- IAA
indole-3-acetic acid
- MS
Murashige and Skoog
- NAA
-naphthaleneacetic acid |
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Keywords: | Embryogenic callus Plant tissueculture Somatic embryos |
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