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Isolation and characterization of purple acid phosphatase gene during seedling development in mungbean |
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| Authors: | A Wongkaew P Srinives S Nakasathien |
| Institution: | 1292. Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand 2292. Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok, 10900, Thailand 3292. Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand 4292. Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand |
| Abstract: | Purple acid phosphatases (PAPs), which are normally found in plant tissues, can hydrolyze a broad spectrum of phosphate esters. In this study, a mungbean Vigna radiata (L.) Wilczek cv. KPS1] acid phosphatase gene (VrPAP1) was isolated from seedling cotyledons. The full-length of VrPAP1 cDNA contained an open reading frame of 1 644 bp encoding 547 amino acid residues with a predicted molecular mass of 62.07 kDa. Sequence analysis showed that VrPAP1 is purple acid phosphatase. RNA blot analyses indicated that the VrPAP1 accumulated during the first hour in cotyledons of germinating seeds and reached a maximum expression after 24 h and then decreased. The VrPAP1 mRNA was observed in cotyledons, hypocotyls and leaves but not in radicles or dry seeds. DNA blot analysis indicated that VrPAP1 is a single copy gene in the mungbean genome. |
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