Proteolytic enzymes from recombinant Streptomyces lividans TK24 |
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| Authors: | Werner Aretz,Klaus P. Koller,Gü nther Riess |
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| Affiliation: | Hoechst AG, Frankfurt/Main, F.R.G. |
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| Abstract: | ![]()
Different proteases from the culture fluids of recombinant Streptomyces lividans strains were isolated. Several individual proteases were separated and characterized. A chymotrypsin-chylike activity (CLA) was identified that specifically degrades a fusion protein between the alpha-amylase inhibitor from S. tendae (Tendamistat, HOE467) and proinsulin from the monkey Macaca fascicularis. The effective chemical inhibition of the degrading enzyme is demonstrated. |
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| Keywords: | α-Amylase inhibitor Tendamistat Chymotrypsin Protein secretion Fusion protein |
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