朱顶红鳞茎芽诱导及植株再生体系的建立

以朱顶红杂交品种红孔雀(Amaryllis vittatacv. Red Peacock)鳞茎盘切块为外植体,对影响鳞茎芽直接萌发增殖及生根的主要因素进行了研究,以建立其高效再生体系.结果表明:(1)1/2MS基本培养基有利于鳞茎芽的萌发和生长;切分方式不同对朱顶红鳞茎增殖具显著效应,偏离切分鳞茎可显著提高增殖系数,最高增殖系数可达6,平均增殖系数为3.6;而对半切分或未切分的增殖系数较低.(2)1/2MS 0.1 mg/L NAA 1.5 mg/L 6-BA 2.0 g/L活性炭的培养基利于鳞茎增殖;活性碳可显著提高鳞茎生根率,在1/2MS 0.1 mg/L NAA 2.0 g/L活性炭的培养基上生根率可达100%,而未加活性炭的生根率较低;再生植株经25 d的自然光照驯化后进行移栽,成活率达90%以上.

Establishment of In vitro Bulb Bud Induction and Regeneration System of Amaryllis vittata

An efficient and reliable method for bulb regeneration from slices of Amaryllis vittata bulb was established.The results showed that 1/2MS was better than MS on bud direct germination;cutting methods had great effect on the multiplication rate of A.vittata bulb.The partial cutting method got the maximum multiplication rate 6 and the average multiplication rate 3.6.While the middle cutting and no cutting method got much lower multiplication rate.The optimum medium for multiplication was 1/2MS 0.1 mg/L NAA 1.5 mg/L 6-BA 2.0 g/L activated carbon.The activated carbon could effectively promote rooting(100%) no matter whether 6-BA were added,1/2MS 0.1 mg/L NAA 2.0 g/L activated carbon was the optimum medium for rooting.The plantlets were transplanted after 25 day,domestication and the survival rate were more than 90%.