| 丝裂原活化的细胞外信号调节激酶在金黄色葡萄球菌诱导的U937细胞凋亡中的作用 |
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| 引用本文： | 王佳贺,胡荣,王鑫,牛慧彦,何平.丝裂原活化的细胞外信号调节激酶在金黄色葡萄球菌诱导的U937细胞凋亡中的作用[J].中国组织化学与细胞化学杂志,2008,17(6). |
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| 作者姓名： | 王佳贺 胡荣 王鑫 牛慧彦 何平 |
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| 作者单位： | 1. 中国医科大学附属盛京医院老年病科
2. 中国医科大学附属盛京医院血液科,沈阳,110004 |
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| 摘 要： | 目的探讨细胞外信号调节激酶（ERK1／2）在金黄色葡萄球菌（简称金葡菌）诱导的人巨噬细胞系U937细胞凋亡中的作用。方法采用AnnexinVFITc／PI双染流式细胞仪检测U937细胞凋亡，用Westernblotting方法分析不同作用时间MEK和ERK1／2的磷酸化水平。预先用不同浓度的PD98059（ERK途径抑制剂）处理U937细胞1h，观察金葡菌感染30min后U937细胞的凋亡情况。结果U937细胞经过金葡菌处理后，发生凋亡，细胞凋亡率呈时间依赖性升高；随着感染时间的延长，MEK和ERK1／2的磷酸化水平逐渐增加，尤以ERK2比较明显。U937细胞的凋亡可被PD98059抑制。结论金葡菌以时间依赖的方式诱导U937细胞凋亡；金葡菌诱导U937细胞凋亡的效应与激活ERK1／2信号转导通路有关。
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| 关 键 词： | 金葡菌 细胞外信号调节激酶 细胞凋亡 |
ROLES OF MITOGEN-ACTIVATED ERK PATHWAYS IN STAPHYLOCOCCUS AUREUS-INDUCED APOPTOSIS IN U937 CELLS |
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| Wang Jiahe,Hu Rong,Wang Xin,Niu Huiyan,He Ping.ROLES OF MITOGEN-ACTIVATED ERK PATHWAYS IN STAPHYLOCOCCUS AUREUS-INDUCED APOPTOSIS IN U937 CELLS[J].Chinese Journal of Histochemistry and Cytochemistry,2008,17(6). |
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| Authors: | Wang Jiahe Hu Rong Wang Xin Niu Huiyan He Ping |
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| Institution: | Wang Jiahe,Hu Rong1,Wang Xin,Niu Huiyan,He Ping(Department of Geriatrics,Shengjing Hospital,China Medical University,Shenyang 110004,China,1Department of Hematology,China) |
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| Abstract: | Objective To explore the role of extracellular signal-regulated kinase(ERK)in the apoptosis of U937 cells induced by Staphylococcus aureus(S.aureus).Methods Apoptosis rates of U937 cells were observed by Annexin V FITC/PI staining and the phosphorylation of MEK and ERK1/2 was measured by western blotting at different time points.U937 cells were pretreated with different concentrations of PD98059(an inhibitor of the ERK1/2 pathway)for 1 h,and the apoptosis rate of U937 cells was detected.Results Following in... |
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| Keywords: | U937 |
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