Low hydration phase properties of phospholipid mixtures |
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| Authors: | Gary Bryant James M Pope Joe Wolfe |
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| Institution: | (1) School of Physics, The University of New South Wales, Kensington, Australia;(2) Present address: Helmholtz-Institut für Biomedizinische Technik an der RWTH Aachen, Pauwelsstrasse 30, W-5100 Aachen, Federal Republic of Germany |
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| Abstract: | An experimental investigation of the low hydration phase properties of phospholipid mixtures is described. 2H (D2O) NMR, X-ray diffraction and differential scanning calorimetry have been used to elucidate the phase properties of mixtures of the mixed chain phospholipids palmitoyloleoylphosphatidylcholine (POPC) and palmitoyloleoylphosphatidylethanolamine (POPE). At 10% hydration pure POPE exhibited a HII phase above 330 K, a fluid lamellar phase below 315 K, and a minimally hydrated crystalline phase below 300 K. For the 1:1 mixture, the samples exhibited only gel or fluid phases between 270 K and 360 K for hydrations in the range 15% to 30%. Below 15% hydration the mixture exhibited two fluid phases with different repeat spacings, as predicted previously. |
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| Keywords: | Phase separations Membrane dehydration Phospholipid phases Lamellar phase Inverse hexagonal phase Mixed chain lipids Deuterium NMR |
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