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Nature of the binding site around and reactivity of histidine-15 in lysozyme |
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| Authors: | H Yamada F Uozumi A Ishikawa T Imoto |
| Abstract: | Bromoacetamide derivatives having n-alkyl substituents (BrCH2CONH(CH2)nH, 1-n) and carboxyalkyl substituents (BrCH2CONH(CH2)nCOOH, 2-n) react with His-15 in lysozyme exclusively at N epsilon 2 at pH 5.5 and 40 degree C. Kinetic studies suggest that lysozyme has a small hydrophobic pocket that binds these reagents in the vicinity of His-15. |
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