Clone bank and physical and genetic map of potato chloroplast DNA |
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Authors: | S Heinhorst G C Gannon E Galun L Kenschaft A Weissbach |
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Institution: | (1) Roche Institute of Molecular Biology, Roche Research Center, 07110 Nutley, NJ, USA;(2) The Weizmann Institute of Science, 76100 Rehovot, Israel;(3) Present address: Department of Chemistry, University of Southern Mississippi, Southern Station, Box 5043, 39406 Hattisburg, Ms, USA |
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Abstract: | Summary Clone banks of PvuII, BamHI and XhoI fragments were generated of the Solanum tuberosum cv Katahdin plastome. These clone banks, in conjunction with molecular hybridization to tobacco ctDNA probes, were used to construct a physical map of potato ctDNA. The potato plastome was found to be a circular molecule of 155–156 Kbp containing two inverted repeat regions of 23–27 Kbp. The arrangement of restriction sites is very similar to that of other Solanaceae plastomes. Heterologous hybridization to known ctDNA encoded gene probes from tobacco allowed us to establish a genetic map of the potato chloroplast genome. The arrangement of these genes on the potato plastome resembles that on most higher plant ctDNAs. |
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Keywords: | Solanum tuberosum Potato plastome Chloroplast DNA DNA restriction profile Restriction endonucleases |
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