Isolation of microsatellite markers in the Emys orbicularis complex and development of multiplex PCR amplification |
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| Authors: | I Pedall H Schäfer U Fritz M Wink |
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| Institution: | (1) Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, INF 364, 69120 Heidelberg, Germany;(2) Museum of Zoology (Museum für Tierkunde), Natural History State Collections, A.B. Meyer Building, 01109 Dresden, Germany |
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| Abstract: | We report the development of 15 new microsatellite markers for Emys orbicularis and Emys trinacris. A survey of 20 individuals showed that all loci are highly polymorphic with 3–14 alleles per locus. Additionally, 22 Glyptemys muhlenbergii primers were checked for cross-species amplification, with 14 being amplified successfully and polymorphic (2–14 alleles).
A set of eight markers was selected and combined into two multiplex PCRs. Levels of genetic diversity were assessed in 648
individuals covering the complete distribution area. The number of alleles ranged from 13 to 24 and observed heterozygosity
varied between 0.515 and 0.852. |
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| Keywords: | Microsatellites Primers Multiplex PCR Emys orbicularis Emys trinacris |
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