A monoclonal antibody specific for prophase phosphorylation of histone deacetylase 1: a readout for early mitotic cells |
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| Authors: | Chiara V Segré Silvia Senese Sara Loponte Stefano Santaguida Paolo Soffientini Gabriela Grigorean |
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| Institution: | 1. These authors equally contributed to the work;2. Department of Experimental Oncology;3. European Institute of Oncology;4. Via Adamello 16;5. 20139 Milan, Italy;6. Present addresses: Fondazione Umberto Veronesi;7. Piazza Velasca 5;8. 20121 Milano, Italy;9. Department of Chemistry and Biochemistry;10. University of California;11. Los Angeles;12. Los Angeles, CA 90095, USA;13. Department of Experimental Oncology;14. Koch Institute for Integrative Cancer Research at MIT;15. Massachusetts Institute of Technology;16. Cambridge, USA;17. IFOM;18. the FIRC Institute for Molecular Oncology Foundation;19. via Adamello 16;20. Chromatography and Mass Spectrometry Division;21. Thermo Fisher Scientific;22. 1400 Northpoint Parkway;23. Suite 10;24. West Palm Beach;25. Fl 33407, USA |
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| Abstract: | Histone deacetylases (HDACs) are modification enzymes that regulate a plethora of biological processes. HDAC1, a crucial epigenetic modifier, is deregulated in cancer and subjected to a variety of post-translational modifications. Here, we describe the generation of a new monoclonal antibody that specifically recognizes a novel highly dynamic prophase phosphorylation of serine 406-HDAC1, providing a powerful tool for detecting early mitotic cells. |
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| Keywords: | antibody cell cycle checkpoint HDAC1 HDAC2 HDACs mitosis phosphorylation post-translational modifications prophase |
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