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| 仙人掌多糖对蛋白质糖基化终产物和醛糖还原酶的抑制效应 | |
| 引用本文： | 杨宁,赵谋明,杨宝,朱邦豪,方芳.仙人掌多糖对蛋白质糖基化终产物和醛糖还原酶的抑制效应[J].天然产物研究与开发,2007,19(4):568-571. |
| 作者姓名： | 杨宁 赵谋明 杨宝 朱邦豪 方芳 |
| 作者单位： | 1. 华南理工大学轻工与食品学院,广州,510640 2. 中国科学院华南植物园,广州,510650 3. 中山大学基础医学院,广州,510080 |
| 基金项目： | The project was supported by Guangdong Provincial Scientific Research Foundation（No.2005B20401024） and Guangzhou Scientific Research Foundation（No.2004Z1-E0061）.Acknowledgements The financial support was provided by Guangdong Provincial Scientific Research Foundation （ No. 2005B20401024 ） and Guangzhou Scientific Research Foundation （ No. 2004Z1-E0061 ） was greatly appreciated. |
| 摘 要： | 以仙人掌为原料提取多糖产品,通过超滤浓缩和不同浓度乙醇分级沉淀,分别得到55%乙醇沉淀的多糖(OPMC I),进一步乙醇浓度增至80%沉淀的多糖(OPMCⅡ),以及一次性80%乙醇沉淀的多糖(OPMCⅢ)。比较了三种多糖对蛋白质非酶糖基化反应的终端产物(AGEs)和醛糖还原酶(AR)形成的抑制作用。结果表明,在对AGEs形成的抑制过程中,第四周时OPMC II的抑制作用较强,且强于同剂量的氨基胍;在对AR活性的抑制作用中,OPMC II在三种多糖样品中抑制作用最强,但明显低于阳性对照物依帕司他。 |
| 关 键 词： | 仙人掌 多糖 糖基化终产物 醛糖还原酶 抑制 |
| 文章编号： | 1001-6880（2007）04-0568-04 |
| 收稿时间： | 2006-10-31 |
| 修稿时间： | 2007-11-27 |
Inhibition of Polysaccharides from Opuntia monacantha on Development of Advanced Glycated End Products and Activity of Aldose Reductase |
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| YANG Ning,ZHAO Mou-ming,YANG Bao,ZHU Bang-hao,FANG Fang.Inhibition of Polysaccharides from Opuntia monacantha on Development of Advanced Glycated End Products and Activity of Aldose Reductase[J].Natural Product Research and Development,2007,19(4):568-571. | |
| Authors: | YANG Ning ZHAO Mou-ming YANG Bao ZHU Bang-hao FANG Fang |
| Abstract: | Polysaccharides from Opuntia monacantha Cladode (OPMC) were extracted by distilled water and concentrated by ultrafiltration.OPMC I was obtained by precipitation OPMC extracts with 55% of ethanol solution.The supernatant was added ethanol to final concentration 80% to precipitate OPMCⅡ.OPMCⅢ was obtained by precipitation OPMC extracts with 80% of ethanol solution directly.The inhibition of three OPMC polysaccharides on the development of advanced glycated end product (AGE) of protein non-enzymaticaly glycated reaction and aldose reductase (AR) activity were investigated.OPMCⅡ showed stronger inhibition effect on AGE than aminoguanidine with the same dose at fourth week.Among OPMC Ⅰ-Ⅲ,OPMCⅡ also showed the strongest inhibition effect on AR activity,but lower than epalrestat,the positive control. |
| Keywords: | Opuntia polysaccharide advanced glycation end-products aldose reductase inhibition |
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