蓝斑区去甲肾上腺素能神经元在Orexin促麻醉觉醒中的作用研究

目的：探讨蓝斑区（LC）去甲肾上腺素能神经元在orexin促麻醉觉醒中作用。方法：应用异氟烷对成年SD大鼠进行麻醉,15分钟后,将SD大鼠随机分为6组,分别注射orexin-A/B（100pmol/0.3μL）及其溶剂saline（0.3μL）;orexin I型受体拮抗剂SB334867/II型受体拮抗剂TCS-OX2-29（20μg/0.3μL及其溶剂DMSO（0.3μL）,通过观察大鼠翻正反射的消失和恢复时间,研究蓝斑区微注射orexin及其拮抗剂对异氟烷麻醉的诱导和觉醒的影响。结果：蓝斑区（LC）微注射四种试剂或其溶剂均对SD大鼠异氟烷麻醉的诱导时间无明显影响;蓝斑区（LC）微注射orexin-A能缩短SD大鼠异氟烷麻醉觉醒时间（P〈0.001）,而微注射orexinI型拮抗剂SB334867能延长觉醒时间（P〈0.001）;orexin-B、orexin II型受体拮抗剂TCS-OX2-29对大鼠异氟烷麻醉的觉醒无明显影响。结论：蓝斑区（LC）的去甲肾上腺素能神经元介导了orexin的促麻醉觉醒作用。

Activation of Norepinephrine Neurons in Locus Coeruleus Mediates the Promotion Effect of Orexin on Anesthesia Emergence

Objective：To investigate the role of norepinephrine neurons of the locus coeruleusin in the promotion effect of orexin in emergence of isoflurane anesthesia.Methods：Isoflurane（ISO） was used to anesthetize adult male SD rats,15 minutes later,the rats were assigned into six groups,Orexin-A,orexin-B（100pmol/0.3 μL） and their solvent saline（0.3 μL）,orexin receptor I antagonist SB334867,orexin receptor II antagonist TCS-OX2-29（20 μg/0.3 μL for both） and their solvent DMSO（0.3 μL） were bilaterally microinjected into LC,respectively.The time of the loss of righting reflex（LORR） following anesthesia was recorded as induction time and the recovery of righting reflex（RORR） from cease of anesthesia were recorded as emergence time.Results：There was no different effect on the induction time of ISO by any of above six microinjections（P〉0.05）.Microinject orexin-A into LC significantly shortened the emergence time and orexin-A receptor I antagonis SB334867 prolonged the emergence time of ISO anesthesia（P〈0.001）.Conclusion：The norepinephrine neurons of LC at least partly mediated the promotion effect of orexin in anesthesia emergence.