错配修复蛋白hMSH2、hMLH1在涎腺粘液表皮样癌中的表达

目的:探讨人类错配修复基因2 the human mutS homolog 2(hMSH2)人类错配修复基因1human mutL homolog 1(hMLH1)在涎腺粘液表皮样癌(salivary gland mucoepidermoid carcinoma-SMEC)表达水平及临床病理意义。重点研究人类错配修复基因2和人类错配修复基因1与目标肿瘤发生的相关性。方法:采用HE染色方法筛选共计47例典型病例,采用免疫组织化学染色分析37例SMEC、10例正常组织涎腺中hMSH2、hMLH1的表达水平,结合计算机辅助高清晰图像分析处理技术做出综合评价。结果:hMLH1的表达与SMEC分化呈负相关(P0.05);hMLH1的低表达或表达缺失在低分化的SMEC中较普遍,在中分化和高分化中表达逐步增强;hMSH2的表达与SMEC分化不相关(P0.05)。结论:hMSH2、hMLH1异常表达与涎腺黏液表皮样的发生、演进存在相关性,以hMLH1、hMSH2为切入点为涎腺黏液表皮样癌治疗与预防提供参考依据。

Expression of hMLH1, hMSH2 in Salivary Gland Mucoepidermoid Carcinoma*

Objective： The aim of this study was to describe the human mutS homolog 2 （hMSH2） and human mutL homolog 1 （hMLH1） immunoexpression in different areas of salivary gland mucoepidermoid carcinoma in an attempt to verify if the histological grade of malignancy interferes in this expression. The research emphasis is whether hMSH2, hMLH1 correlate with target carcinoma. Methods： Tissues in 37 cases with mucoepidermoid carcinoma and 10 normal salivary gland were determined by HE staining and the ex- pression of hMLH1, hMSH2 were tested using computer-assised high-resolution image analysis and processing techniques. Results： The hMHL 1 immunoexpression was negatively correlated with histological grades of malignancy（P〈0.05 ）; hMHL 1 reduction or negative ex- pression was detected in poorly differentiated tumors, but the expression gradually enhanced in moderately differentiated and well differ- entiated tumors. The hMSH2 immunoexpression was not correlated with histological grades of malignancy, whereas hMLH1 overexpres- sion was correlated and associated （P〉0.05） with histological grades of malignancy. Conclusion： The anomalo expression of hMLH1, hMSH2 get the relationship with the occurrence and development of slivary gland mucoepidermoid carcinoma. We can use hMLH1, hMSH2 as a point to incisivus in slivary gland tumour, and they have graveness significance on the precaution and therapy to slivary gland mucoepidermoid carcinoma.