Production of polysaccharides by liquid cultures of Polianthes tuberosa cells; high production by reduction in the viscosity of culture medium |
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Authors: | Akira Takei Yutaka Shoga Masakatu Hama Yasuki Honda Yukio Sugimura Kazuya Otsuji |
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Institution: | (1) Kashima Research Laboratories, Kao Corporation, Kashima, 314-02 Ibaraki, Japan;(2) Tochigi Research Laboratories, Kao Corporation, Haga, 321-34 Tochigi, Japan |
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Abstract: | Summary To improve the production of extracellular polysaccharides (EPS) in liquid cultures of Polianthes tuberosa (tuberose) cells, the viscosity of the culture medium was lowered by addition of mineral salts. In cultures in the medium supplemented with 30 mM CaCl2, higher production of EPS (6.5g/l) has been realized (vs. 4.6 g/l without CaCl2). |
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