Identification of an heterologous histone complex using reversible crosslinking

Using formaldehyde as a reversible crosslinker, it has been shown that an heterologous dimer is the principal components in a mixture of calf thymus H2A and sea urchin H2B₁. Comparison of the crosslinking ability of formaldehyde with dimethyl suberimidate demonstrates that suberimidate gives a better representation of the free-solution equilibrium of the protein mixture.