Direct measurement of diffusion in olfactory cilia using a modified FRAP approach |
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| Authors: | Mihai Alevra Peter Schwartz Detlev Schild |
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| Affiliation: | 1. Department of Neurophysiology and Cellular Biophysics, University of Göttingen, Göttingen, Germany.; 2. German Research Foundation Research Center for Molecular Physiology of the Brain, Göttingen, Germany.; 3. German Research Foundation Cluster of Excellence 171, Göttingen, Germany.; 4. Department of Anatomy and Embryology, University of Göttingen, Göttingen, Germany.;German Cancer Research Center, Germany |
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| Abstract: | The diffusion coefficient of fluorescein in detached cilia of Xenopus laevis olfactory receptor neurons was measured using spatially-resolved FRAP, where the dye along half of the ciliary length was photobleached and its spatiotemporal fluorescence redistribution recorded. Fitting a one-dimensional numerical simulation of diffusion and photobleaching for 35 cilia resulted in a mean value of the diffusion coefficient (1.20 ± 0.23) · 10(-10)m(2)/s and thus a reduction by a factor of 3.4 compared to free diffusion in aqueous solution. |
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