Arabidopsis consensus intron sequences |
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| Authors: | John W S Brown Philip Smith Craig G Simpson |
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| Institution: | (1) Department of Cell and Molecular Genetics, Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, Scotland, UK;(2) Department of Data Processing, Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, Scotland, UK |
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| Abstract: | We have analysed 998 Arabidopsis intron sequences in the EMBL database. All Arabidopsis introns to adhere to the :GU...AG: rule with the exception of 1% of introns with :GC at their 5 ends. Virtually all of the introns contained a putative branchpoint sequence (YUNAN) 18 to 60 nt upstream of the 3 splice site. Although a polypyrimidine tract was much less apparent than in vertebrate introns, the most common nucleotide in the region upstream of the 3 splice site was uridine. Consensus sequences for 5 and 3 splice sites and branchpoint sequences for Arabidopsis introns are presented. |
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| Keywords: | pre-mRNA splicing introns splice site branchpoint |
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