Ammonium carboxylate production from sugarcane trash using long-term air-lime pretreatment followed by mixed-culture fermentation |
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Authors: | Nachiappan Balasubramaniyan Fu Zhihong Holtzapple Mark T |
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Affiliation: | a Department of Chemical Engineering, 3122 TAMU, Texas A&M University, College Station, TX 77843-3122, USA b Florida Center for Renewable Chemicals and Fuels (FCRC), Department of Microbiology and Cell Science, P.O. Box 110700, University of Florida, Gainesville, FL 32611-0700, USA |
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Abstract: | ![]() Sugarcane trash (ST) was converted to ammonium carboxylates using a novel bioprocessing strategy known as long-term air-lime pretreatment/mixed-culture fermentation. At mild conditions (50 °C, 5 weeks, 1-atm air, and excess lime loading of 0.4 g Ca(OH)2/(g dry biomass)), air-lime pretreatment of ST had moderate delignification (64.4%) with little loss in polysaccharides. Without employing detoxification, sterility, expensive nutrients, or costly enzymes, the feedstock (80% treated ST/20% chicken manure) was fermented to primarily ammonium acetate (>75%) and butyrate by a mixed culture of marine microorganisms at 55 °C. In the best four-stage countercurrent fermentation, the product yield was 0.36 g total acids/(g VS fed) and the substrate conversion was 64%. Model predictions indicate both high acid concentrations (>47.5 g/L) and high substrate conversions (>70%) are possible at industrial scale. |
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Keywords: | Sugarcane trash Air-lime pretreatment Mixed-culture fermentation Biocrude Ultimate consolidated bioprocessing |
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