肝郁脾虚泄泻大鼠模型的建立及评价

目的采用多因素刺激方法建立吻合人类"肝郁脾虚泄泻"疾病特征的大鼠模型,并选取操作简单、结论实时、科学经济的评价指标。方法将幼年SD大鼠随机分为空白组和模型组,模型组采用母婴分离、束缚刺激、直肠醋酸刺激三种因素复合造模,选择不同时间测定大鼠体重变化、直肠敏感性、大鼠粪便分型积分及粪便含水量等指标,客观验证肝郁脾虚泄泻模型是否成功。结果模型大鼠出现食欲减退,饮水量增大,尿液减少的病理现象,部分大鼠出现活动增加、甚至躁狂。粪便积分在5~7分,粪便含水量远远高于正常组大鼠,而肠道敏感性显著增加。结论该模型较好的模拟了肝郁脾虚泄泻的临床表现,能满足该类疾病的相关研究。

Establishment and assessment of the diarrhea rat model of liver-QI stagnation with spleen deficiency

Objective To establish a diarrhea rat model using multiple-stimulating factors and choosing the best indexes to assess whether the model is consistent with the disease characteristics of liver -QI stagnation with spleen deficien-cy in traditional Chinese medicine .Methods Newborn SD rats were randomly divided into model group （ n=20 ） and control group （n=10）.The rats of model group were stimulated by maternal separation , restraint stress and rectum acetic acid irritation, while the rats in control group were fed as normal .Weight changes, rectal sensitivity, Bristol scores and wa-ter content of feces and histology of the colon tissues were used as evaluation indexes to assess whether the model meets the demands for further studies .Results The rats in the model group showed loss of appetite , increase of water intake and u-rine reduction .Some rats showed increased activity , and even mania .Bristol scores and water content of feces were signifi-cantly higher than that of the control group , and the rectal sensitivity was significantly increased .The colon mucosa showed slightly thickened submucosal layer and mild inflammatory cell infiltration in the model rats .Conclusions The rat model established in this study is better to simulate the clinical manifestation of liver -QI stagnation with spleen deficiency in Chinese medicine , and may meet the demands of related researches of this disease .