A Chromatographic Method for Analysis of the Gaseous Phase in Shake Flask Cultures and its Application to the Study of Methane Utilization |
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| Authors: | P M Molton S J Pirt |
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| Institution: | Department of Microbiology, Queen Elizabeth College, Campden Hill, London W8, England |
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| Abstract: | A simple gas chromatograph with a katharometer detector is described to determine O2, N2, methane and CO2 in gas samples of 0·01–2·0 ml. The apparatus is inexpensive, and can be modified to determine other gases. The sensitivity to oxygen is 3 × 10?6 g. The use of the instrument is illustrated by a study of the growth kinetics of Methylococcus capsulatus grown on methane in shake flask experiments. The ratio of O2 uptake to methane uptake is much lower in the stationary phase than in the growth phase of the culture. |
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