Characteristic properties of metabolism of the yeast <Emphasis Type="Italic">Yarrowia lipolytica</Emphasis> during the synthesis of α-ketoglutaric acid from ethanol |
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Authors: | A P Ilchenko V Ya Lysyanskaya T V Finogenova I G Morgunov |
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Institution: | 1.Skryabin Institute of Biochemistry and Physiology of Microorganisms,Russian Academy of Sciences,Pushchino, Moscow oblast,Russia |
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Abstract: | The study of free amino acid content in Yarrowia lipolytica cells grown on ethanol under thiamine deficiency showed that glutamate, alanine, and γ-aminobutyric acid (γ-ABA) occurred
in the highest concentrations among the present 17 free amino acids. The culture liquid contained no amino acids. Analysis
of the enzymes of oxidative metabolism in the yeast grown under these conditions showed that the cell-free homogenate contained
substantial activity of glutamate decarboxylase, γ-ABA transaminase, and succinyl semialdehyde dehydrogenase. This result
indicated the formation of succinate from glutamate in a reaction catalyzed by 4-aminobutyrate aminotransferase (γ-aminobutyrate
bypass) under severe thiamine deficiency. These studies lead to the conclusion that cultivation of the yeast Y. lipolytica on ethanol under thiamine deficiency causes adaptive stress-induced metabolic changes. Increase of ammonium nitrogen consumption
and excretion of α-ketoglutaric acid are indicative of physiological changes, the functioning of the γ-aminobutyrate bypass
and high activity of malate dehydrogenase are manifestations of metabolic changes, and increased activities of the transamination
reactions reflect the changes in nitrogen metabolism. |
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Keywords: | |
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