T-DNA presence and opine production in tumors of Picea abies (L.) Karst induced by Agrobacterium tumefaciens A281 |
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| Authors: | Elizabeth E Hood David H Clapham Inger Ekberg Thomas Johannson |
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| Institution: | (1) Dept. of Forest Genetics, Swedish University of Agricultural Sciences, Box 7027, S-750 07 Uppsala, Sweden;(2) Present address: Department of Biology, Utah State University, 84322-5305 Logan, Utah, USA |
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| Abstract: | The hypervirulent Agrobacterium tumefaciens strain A281 formed frequent tumors (31%) on Picea abies (Norway spruce), an economically important tree species in Swedish forests. Three-month-old seedlings were inoculated and tumors were established that grew hormone-independently in culture. Tumors contained agropine and mannopine/mannopinic acid as determined by acid pH paper electrophoresis. In addition, DNA hybridization studies showed that the DNA from these tumor lines contained sequences homologous to Ti plasmid T-DNA, whereas wild-type spruce seedling DNA did not. These results suggest that Agrobacterium vectors can be used for gene transfer into this important forest species. |
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| Keywords: | Agrobacterium conifers DNA hybridization transformation |
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