Antifungal and other Biological Activities from Oudemansiella
Canarii(Basidiomycota) |
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Authors: | Luiz H Rosa Betania B Cota Kátia M G Machado Carlos A Rosa Carlos L Zani |
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Institution: | 1. Laboratório de Química de Produtos Naturais, CPqRR, Funda??o Oswaldo Cruz, Av. Augusto de Lima, 1715, 30190 002, Belo Horizonte, Brasil 3. Funda??o centro Tecnológico de Minas Gerais, CETEC, av. José Candido da Silveira 2000, Belo Horizonte, Brasil 2. Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Caixa Postal 486, 30123-970, Belo Horizonte, MG, Brasil
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Abstract: | Summary The ethyl acetate extract from the fungus Oudemansiella canarii grown in malt extract medium was evaluated against (a) the recombinant enzyme trypanothione reductase from Trypanosoma cruzi, (b) lymphocyte proliferation in human peripheral blood mononuclear cells (PBMCs) stimulated with phytohaemaglutinin, (c)
the human tumour cell lineages MCF-7, TK-10 and UACC-62, and (d) the phytopathogenic fungus Cladosporium sphaerospermum. At 10 μg/ml, the crude extract was inactive against PBMC but inhibited the growth of UACC-62 cells by 47% and the enzyme
trypanothione reductase (TryR). It also presented strong inhibition in the bioautographic assay with C. sphaerospermum. Chromatographic fractionation guided by this assay allowed the isolation of oudemansin A (1), a known fungitoxic compound
that showed a minimum inhibitory concentration (MIC) of 1.25 μg/spot in the bioautographic assay. As oudemansin A was not
active in the other assays, other components in the extract may be responsible for the observed activities by the crude extract
against the UACC-62 cells or the TryR enzyme. |
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Keywords: | Basidiomycetes cancer cells lymphocytes oudemansin A PBMC phytopathogens Trypanosoma cruzi |
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