Cloning and over-expression of an alkaline protease from Bacillus licheniformis |
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| Authors: | Xue-Ming Tang Wei Shen F.M. Lakay Wei-Lan Shao Zheng-Xiang Wang B.A. Prior Jian Zhuge |
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| Affiliation: | The Key Laboratory of Industrial Biotechnology, Ministry of Education, Research Center of Industrial Microbiology, Southern Yangtze University, Wuxi 214036, P.R. China. txm@sun.ac.za |
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| Abstract: | The alkaline protease gene, apr, from Bacillus licheniformis 2709 was cloned into a Bacillus shuttle expression vector, pHL, to yield the recombinant plasmid pHL-apr. The pHL-apr was expressed in Bacillus subtilis WB600, yielding a high expression strain BW-016. The amount of alkaline protease produced in the recombinant increased by 65% relative to the original strain. SDS-PAGE analysis indicated a Mr of 30.5 kDa. The amino acid sequence deduced from the DNA sequence analysis revealed a 98% identity to that of Bacillus licheniformis 6816. |
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| Keywords: | alkaline protease Bacillus licheniformis over-expression pro-sequence subtilisin |
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