Extractive bioconversions in aqueous two-phase systems: enzymatic hydrolysis of casein proteins |
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Authors: | Mukataka S Haynes C A Prausnitz J M Blanch H W |
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Affiliation: | Department of Chemical Engineering, University of California, Berkeley, California 94720. |
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Abstract: | Partition coefficients in poly(ethylene glycol)/dextran aqueous two-phase systems are reported for mixed-casein and its components, alpha, beta and kappa casein. Rates of casein proteolysis by alpha-chymotrypsin and by trypsin are reported in single-phase and aqueous two-phase reactor systems. The advantages resulting from selective partitioning of substrates, enzymes, and products are examined in terms of relative volumetric reaction rates. |
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Keywords: | bioconversions hydrolysis casein proteins catalysis |
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