Protein export during growth of Bacillus subtilis: the effect of extracellular protease deficiency |
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Authors: | RD Coxon CR Harwood AR Archibald |
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Institution: | Department of Microbiology, The Medical School, Framlington Place, Newcastle upon Tyne NE2 4HH, UK |
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Abstract: | Exponential phase cultures of a protease-deficient and a protease-proficient strain of Bacillus subtilis , growing in minimal medium with a glucose carbon source, were labelled with 14C-phenylalanine. Subsequent export of labelled protein was detected in trichloroacetic acid-precipitated supernatant fluid samples. During exponential growth the strains exhibited substantially similar protein export kinetics. However, as confirmed by the presence of a cytoplasmic enzyme in the culture supernatant fluid, protease deficiency was associated with an increased tendency of cells to lyse as they approached stationary phase. |
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