The hepatoprotective effect of coumarin and coumarin derivates on carbon tetrachloride-induced hepatic injury by antioxidative activities in rats |
| |
Authors: | Mukadder Atmaca Hakkı Murat Bilgin Basra Deniz Obay Hüda Diken Mustafa Kelle Ebru Kale |
| |
Institution: | (1) Department of Physiology, Dicle University Faculty of Medicine, 21280 Diyarbakır, Turkey;(2) Department of Biochemistry, Dicle University Faculty of Medicine, Diyarbakır, Turkey; |
| |
Abstract: | Coumarins are a vast group of natural compounds and some of them possess antioxidant activities. The comparison of the antioxidant
activity of some coumarins with various chemical molecular structure has not been investigated in previous studies. Therefore,
this study was aimed to investigate the hepatoprotective effect against carbon tetrachloride (CCl4) -induced hepatic injury by coumarin (1,2-benzopyrone) and coumarin derivatives, esculetin (6,7-dihydroxycoumarin), scoparone
(6,7-dimethoxycoumarin), and 4-methylumbelliferone (7-hyroxy-4-methyl) in male Sprague–Dawley rats. Product of lipid peroxidation,
malondialdehyde (MDA), activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) were evaluated for oxidative
stress in hepatic injury. Gamma glutamyl transpeptidase (GGT), lactate dehydrogenase (LDH) were detected in plasma as a biomarker
of hepatic injury. Significantly elevated levels of MDA and lowered levels of SOD and CAT activities were observed in liver
of rats exposed to CCl4, when compared to control values. Similarly, administration of CCl4 increased LDH and GGT levels in serum. Pre-treatment of rats with esculetin (35 mg kg−1, orally) and scoparone (35 mg kg−1, orally) significantly prevented CCl4-induced decrease in MDA levels and increase in SOD and CAT, whereas 4-methylumbelliferone (35 mg kg−1) and coumarin (30 mg kg−1) had no effect against CCl4-induced rise in serum enzymes. Esculetin and scoparone also showed protective properties as was evidenced in reduced LDH
and GGT levels in serum. The results of this study indicate that the chemical structures of coumarins play an important role
in the prevention of oxidative stress. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|