混合发酵提高2株海洋微生物菌株抑菌活性的研究

2株抑菌范围不同的海洋微生物菌株，在单独培养条件优化基础上进行混合发酵，并对混合发酵条件进行了优化测试，混合发酵结果有效的提高了菌株产生代谢产物的抑菌活性，最小抑菌浓度由单独发酵的156μL/mL、125μL/mL，单独发酵后混合的250μL/mL、218μL/mL降至32μL/mL、28μL/mL，即代谢产物抑菌活性比单独发酵提高200%，比单独发酵后混合提高300%，2株菌株混合发酵的协同效应大于单独发酵混合后的累加效应，对靶标真菌的致畸作用明显。

Study on increasing anti-fungi activity of marine microbes by mixed culture

The effect of mixed culture on the anti-fungi activity of marine microbes metabolites was evaluated. Based on optimization of monoculture, orthogonal design was used to approach the optimal region of the medium composition of mixed fermentation. The mixed culture showing anti-fungi activity of marine microbes was enriched obviously, compared with monoculture and fermentation mixture. The MIC (minimal inhibitory concentration) reduced from 156 microL/mL, 125 microL/mL by monoculture and 250 microL/mL, 218 microL/mL by mixture of monoculture fermentation to 32 microL/mL, 28 microL/mL, indicate 200% to 300% increase separately in fungi inhibition with mixed culture.