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Liposome-mediated labeling of adrenocorticotropin fragments parallels their biological activity |
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| Authors: | B Gysin R Schwyzer |
| Institution: | Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule, CH-8093 Zürich, Switzerland |
| Abstract: | To test our hypothesis that specific interactions of ACTH peptides with model lipid membranes reflect the biological importance of similar interactions on target cells, we investigated the liposome-mediated labeling of ACTH fragments with the extremely hydrophobic photolabel, 3-trifluoromethyl-3-(m-125I]iodophenyl)diazirine. Correlations were found between the labeling rates and the agonistic and antagonistic potencies of the peptides for in vitro steroidogenesis and inhibition of a synaptosomal protein kinase. A model for the cross-reactivity between ACTH and opioid peptides is discussed. |
| Keywords: | Peptide hormone—lipid membrane interaction Hydrophobic photolabeling Adrenocorticotropin Dynorphin Enkephalin Endorphin Liposome ACTH adrenocorticotropic hormone fragments of peptides dynorphin-(1–13)-tridecapeptide amino acids according to the IUPAC-IUP recommendations PA dipalmitoylphosphatidic acid PC egg yolk lecithin phosphatidylcholine |
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