A sequence from drosophila DNA cross-hybridizing with a mouse class I H-2 Gene: absence of relevant nucleic acid or amino acid sequence homology |
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Authors: | Alain Israel Jean-Louis Becker Odile Le Bail Philippe Kourilsky |
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Affiliation: | 1. Unité de Biologie Moléculaire du Gène U277 INSERM associée au CNRS (UA 535) Institut Pasteur, 25 rue du Dr. Roux, 75724 Pairs Cedex 15, France |
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Abstract: | Following serological data (1) showing cross-reactivity of drosophila surface antigens with anti-H-2 and mouse beta 2-microglobulin (beta 2-m) antisera, we have looked for homologous sequences in the drosophila genome by low stringency hybridization with mouse H-2 and beta 2-m probes. A 240 bp drosophila DNA segment cross-hybridizing with a H-2 probe, was isolated and sequenced. No homology at the nucleotide or amino acid levels was found with the mouse probe which was used, except for a perfectly matching 16-mer containing 11 G-C, which might be responsible for the cross-hybridization. Therefore, our present data do not support the existence of class I H-2 and/or beta 2-m related gene sequences in the drosophila genome. |
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Keywords: | génome drosophile séquences H-2 et β2-microglobuline de souris hybridation à faible stringence drosophila genome mouse H-2 and β2-microglobuline sequences low stringency hybridization |
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