Efficient micropropagation protocol of <Emphasis Type="Italic">Spilanthes acmella</Emphasis> L. possessing strong antimalarial activity |
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Authors: | Vibha Pandey Veena Agrawal |
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Institution: | (1) Department of Botany, University of Delhi, Delhi, 110007, India |
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Abstract: | Micropropagation has been achieved in a promising larvicidal asteraceous taxon Spilanthes acmella L. using seedling leaf explants. The explants were reared on a variety of growth regulators, namely 2,4-dichlorophenoxyacetic
acid, 1-naphthalene acetic acid, Indole-3-butyric acid, N6-benzyladenine, and kinetin either alone or in combination on Murashige and Skoog’s (MS) medium. The best green and compact
callus was obtained on 1 μM NAA and 10 μM benzyladenine (BA) in 15 d. The callus on subculture to the same but fresh medium
after every 30 d differentiated an average of 12.90 ± 0.32 shoot buds in 50% cultures. Elongation in shoot buds occurred only
if they were transferred to NAA lacking MS+BA medium. An average number of 4.22 ± 0.83 shoots and 15 ± 0.84 shoot buds per
explant were obtained in 70.3% cultures on MS + 10 μM BA in 30 d. One hundred percent excised shoots rooted in MS(1/2) + 0.1 μM
IBA within 2 wk. The plants were gradually hardened and established in soil where they flowered and set viable seeds. The
regenerated plants were morphologically similar to the field grown plants and showed 100% larvicidal activity against malaria
and filarial vectors. |
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Keywords: | BA Larvicidal activity Leaf explant Multiple shoots NAA Plantlets |
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