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Organisation and expression of a wound/ripening-related small multigene family from tomato |
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| Authors: | Michael J Holdsworth Wolfgang Schuch Donald Grierson |
| Institution: | (1) Department of Physiology and Environmental Science, University of Nottingham, School of Agriculture, Sutton Bonington, LE12 5RD Loughborough, UK;(2) Present address: Department of Biology, UCLA, 405 Hilgard Avenue, Los Angeles, California, USA;(3) ICI Plant Biotechnology Group, The Heath, P.O. Box 11, WA7 4QE Runcorn, Cheshire, UK |
| Abstract: | cDNA clones derived from a ripe tomato fruit cDNA library were used to investigate changes in the abundance of specific mRNAs in ripening fruit and wounded leaves. mRNAs related to one cDNA clone (pTOM 13) were expressed in both situations. This clone was used to identify homologous sequences in a tomato genomic library. Three groups of related clones that hybridised to the pTOM 13 cDNA insert were identified and subcloned into plasmid vectors. Genomic Southern analysis of tomato DNA using gene-specific DNA fragments isolated from the subcloned DNAs indicated that all pTOM 13 closely related genes had been isolated. RNA dot blot analysis with these DNA fragments as probes indicated differential expression of this small multigene family in leaves and fruit. |
| Keywords: | wound/ripening-related gene expression |
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