氨基羟乙酸对慢性酒精中毒大鼠学习记忆的影响及可能的机制

目的：探究氨基羟乙酸（AOAA）对慢性酒精中毒大鼠学习记忆能力及可能机制的影响。方法：将60只SD雄性大鼠随机均分为3组（n=20）：空白对照组、模型组和治疗组。模型组和治疗组饮含6%（v/v）酒精水溶液28 d。14 d后，治疗组连续14 d腹腔注射AOAA（5 mg/kg·d）注射液，其余两组注射等量生理盐水。实验结束前5 d连续进行5 d的水迷宫实验，实验结束取大鼠海马组织检测H₂S含量、线粒体ATP酶活性及5-HT受体蛋白的表达。结果：与空白对照组比较，模型组大鼠水迷宫实验的第2~4日潜伏期、第2~4日游泳距离、H₂S含量均升高，ATP酶活性和海马CA1、CA3区5-HT受体阳性表达明显下降（P<0.01）；与模型组比较，治疗组大鼠第2~4日潜伏期、第2~4日游泳距离、H₂S含量均下降，ATP酶活性和海马CA1、CA3区5-HT受体阳性表达显著升高（P< 0.01）。结论：AOAA能够减轻慢性酒精中毒大鼠的症状，可能与AOAA影响H₂S的含量、线粒体酶活性、5-HT受体的含量有关。

Effects of aminooxyacetic acid on the learning and memory ability and its possible mechanism in rats with chronic alcoholism

Objective: To investigate the effects of aminooxyacetic acid (AOAA) on learning and memory ability and possible mechanisms in rats with chronic alcoholism.Methods: Sixty SD male rats were randomly divided into three groups on average.The model group rats and the remedy group rats were fed with the water containing (v/v) 6% alcohol for 28 days.After 14 days, the remedy group rats were treated with AOAA (5 mg/kg·d) by intraperitoneal injection once a day for 14 days and the other two group rats were treated with the equal amount of saline by intraperitoneal injection every day.Five days before the end of the experiment, the water maze test was carried out to test the learning and memory ability of rats for 5 days.Subsequently, the content of H₂S, the activity of ATP enzyme and the expression of 5-HT in hippocampus were measured.Results: Compared with the rats in the control group, the latency and the swimming distance of the 2nd to the 4th day, the content of H₂S in hippocampus of rats in the model group were all increased, the mitochondrial ATP enzyme activity in hippocampus and the positive expression of 5-HT in hippocampus CA1 and CA3 of rats in the model group were decreased (P<0.01).Compared with the rats in the model group, the latency and the swimming distance of the 2nd to the 4th day, the content of H₂S in hippocampus of the rats in the remedy group were decreased, the mitochondrial ATP enzyme activity in hippocampus and the positive expression of 5-HT in hippocampus CA1 and CA3 of rats in the model group were increased (P<0.01).Conclusion: AOAA could alleviate the symptoms of chronic alcoholism rats, which may be related to the effects of AOAA on the content of H₂S, the mitochondrial enzyme activity and the expression of 5-HT in hippocampus.